Teaching
- - Teaching basic molecular and cell biology and laboratory techniques to post graduate students/keuzeonderwijs/Pharmacy trainee students
- - Tutor for 1st year medical students (since 2001), Erasmus University Medical Centre Curriculum
- - Regular teacher for 1st year medical students (Thema 1.2: Circulatie, regulatie en Hemodynamiek)
- - Teacher for 3rd year medical students (Thema 3.2: Myasthenia gravis)
- - Teacher in COEUR Courses: #2 (Cardiovascular pharmacology) & #9 (Congenital Heart Disease)
- - Teaching of basic Pharmacology to 2nd year Medical students (1994-2001), Erasmus MC Curriculum
- - Visiting teacher for Molecular Biology and Pharmacology to
undergraduate/graduate students at Dr. B.R. Ambedkar Centre for
Biomedical Research, Delhi University, India
Technologies
Expertise in the following Biochemical, Pharmacological and Molecular Biological techniques:
- extraction and purification of cellular RNA
- in vitro translation, synthesis and cloning of cDNA construction of cDNA libraries, isolation of plasmid/phage DNA, and identification of recombinant clones.
- DNA sequencing and analysis, autoradiography, Northern and Southern blotting, differential hybridization, subtractive cDNA cloning, expression of cDNAs in bacteria and purification of recombinant protein
- Transfection and generation of stable mammalian cell lines, Polymerase Chain Reaction, DD-RT-PCR, in situ hybridisation, nuclear run on assay, DNA microarray analysis, isolation, culture and characterization of smooth muscle/endothelial cells, electrophoresis, Western blotting, immunohistochemistry
- in vitro pharmacology (organ bath and Mulvany myograph), colorimetry, ion-exchange column chromatography, gel filtration, paper chromatography, TLC, HPLC, ultra-centrifugation, etc.
- Experienced in handling “genetically modified organisms” as well as “radioisotopes (32P, 35S, 14C, 3H)”